De NovoGenome Assemblies for Three North American Bumble Bee Species:Bombus bifarius,Bombus vancouverensis, andBombus vosnesenskii

Thumbnail Image

Files

PMC7407468-2585.pdf (1.9 MB)

Date

2020

Authors

Journal Title

Journal ISSN

Volume Title

Publisher

Oxford University Press

Abstract

Bumble bees are ecologically and economically important insect pollinators. Three abundant and widespread species in western North America,Bombus bifarius,Bombus vancouverensis, andBombus vosnesenskii, have been the focus of substantial research relating to diverse aspects of bumble bee ecology and evolutionary biology. We presentde novogenome assemblies for each of the three species using hybrid assembly of Illumina and Oxford Nanopore Technologies sequences. All three assemblies are of high quality with large N50s (> 2.2 Mb), BUSCO scores indicating > 98% complete genes, and annotations producing 13,325 - 13,687 genes, comparing favorably with other bee genomes. Analysis of synteny against the most complete bumble bee genome,Bombus terrestris, reveals a high degree of collinearity. These genomes should provide a valuable resource for addressing questions relating to functional genomics and evolutionary biology in these species.

Description

Keywords

Bombus bifarius, Bombus vancouverensis, Bombus vosnesenskii, Illumina, Oxford Nanopore Technologies, hybrid assembly, MaSuRCA, FORAGING DISTANCE, LAND-USE, APIDAE, CONSERVATION, HYMENOPTERA, PATTERNS, ANNOTATION, DIVERGENCE, ALIGNMENT, SEQUENCE, Genetics & Heredity

Citation

Heraghty, S. D., Sutton, J. M., Pimsler, M. L., Fierst, J. L., Strange, J. P., & Lozier, J. D. (2020). De Novo Genome Assemblies for Three North American Bumble Bee Species: Bombus bifarius, Bombus vancouverensis, and Bombus vosnesenskii. In G3 Genes|Genomes|Genetics (Vol. 10, Issue 8, pp. 2585–2592). Oxford University Press (OUP). https://doi.org/10.1534/g3.120.401437

URI

https://ir.ua.edu/handle/123456789/12177

Collections

Research and Publications - Department of Health Science