Microbial Community Responses to Organophosphate Substrate Additions in Contaminated Subsurface Sediments

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dc.contributor.authorMartinez, Robert J.
dc.contributor.authorWu, Cindy H.
dc.contributor.authorBeazley, Melanie J.
dc.contributor.authorAndersen, Gary L.
dc.contributor.authorConrad, Mark E.
dc.contributor.authorHazen, Terry C.
dc.contributor.authorTaillefert, Martial
dc.contributor.authorSobecky, Patricia A.
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherUniversity of California Berkeley
dc.contributor.otherUnited States Department of Energy (DOE)
dc.contributor.otherLawrence Berkeley National Laboratory
dc.contributor.otherUniversity of Tennessee Knoxville
dc.contributor.otherGeorgia Institute of Technology
dc.date.accessioned2023-10-02T15:18:04Z
dc.date.available2023-10-02T15:18:04Z
dc.date.issued2014
dc.description.abstractBackground: Radionuclide-and heavy metal-contaminated subsurface sediments remain a legacy of Cold War nuclear weapons research and recent nuclear power plant failures. Within such contaminated sediments, remediation activities are necessary to mitigate groundwater contamination. A promising approach makes use of extant microbial communities capable of hydrolyzing organophosphate substrates to promote mineralization of soluble contaminants within deep subsurface environments. Methodology/Principal Findings: Uranium-contaminated sediments from the U. S. Department of Energy Oak Ridge Field Research Center (ORFRC) Area 2 site were used in slurry experiments to identify microbial communities involved in hydrolysis of 10 mM organophosphate amendments [i.e., glycerol-2-phosphate (G2P) or glycerol-3-phosphate (G3P)] in synthetic groundwater at pH 5.5 and pH 6.8. Following 36 day (G2P) and 20 day (G3P) amended treatments, maximum phosphate (PO43-) concentrations of 4.8 mM and 8.9 mM were measured, respectively. Use of the PhyloChip 16S rRNA microarray identified 2,120 archaeal and bacterial taxa representing 46 phyla, 66 classes, 110 orders, and 186 families among all treatments. Measures of archaeal and bacterial richness were lowest under G2P (pH 5.5) treatments and greatest with G3P (pH 6.8) treatments. Members of the phyla Crenarchaeota, Euryarchaeota, Bacteroidetes, and Proteobacteria demonstrated the greatest enrichment in response to organophosphate amendments and the OTUs that increased in relative abundance by 2-fold or greater accounted for 9%-50% and 3%-17% of total detected Archaea and Bacteria, respectively. Conclusions/Significance: This work provided a characterization of the distinct ORFRC subsurface microbial communities that contributed to increased concentrations of extracellular phosphate via hydrolysis of organophosphate substrate amendments. Within subsurface environments that are not ideal for reductive precipitation of uranium, strategies that harness microbial phosphate metabolism to promote uranium phosphate precipitation could offer an alternative approach for in situ sequestration.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationMartinez, R. J., Wu, C. H., Beazley, M. J., Andersen, G. L., Conrad, M. E., Hazen, T. C., Taillefert, M., & Sobecky, P. A. (2014). Microbial Community Responses to Organophosphate Substrate Additions in Contaminated Subsurface Sediments. In M. R. Mormile (Ed.), PLoS ONE (Vol. 9, Issue 6, p. e100383). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0100383
dc.identifier.doi10.1371/journal.pone.0100383
dc.identifier.orcidhttps://orcid.org/0000-0002-1618-9827
dc.identifier.orcidhttps://orcid.org/0000-0001-9509-7324
dc.identifier.orcidhttps://orcid.org/0000-0002-2536-9993
dc.identifier.orcidhttps://orcid.org/0000-0001-9973-7798
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12611
dc.languageEnglish
dc.language.isoen_US
dc.publisherPLOS
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPLANT-GROWTH PROMOTION
dc.subjectIN-SITU BIOREDUCTION
dc.subjectPHOSPHATASE-ACTIVITY
dc.subjectURANIUM BIOMINERALIZATION
dc.subjectPHYLOGENETIC ANALYSIS
dc.subjectMETHANOGENIC ARCHAEA
dc.subjectBACTERIAL DIVERSITY
dc.subjectREDUCED URANIUM
dc.subjectREDUCTION
dc.subjectREOXIDATION
dc.subjectMultidisciplinary Sciences
dc.titleMicrobial Community Responses to Organophosphate Substrate Additions in Contaminated Subsurface Sedimentsen_US
dc.typeArticle
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