Absolute Oxygen R-1e Imaging In Vivo with Pulse Electron Paramagnetic Resonance

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dc.contributor.authorEpel, Boris
dc.contributor.authorBowman, Michael K.
dc.contributor.authorMailer, Colin
dc.contributor.authorHalpern, Howard J.
dc.contributor.otherUniversity of Chicago
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T22:02:17Z
dc.date.available2023-09-28T22:02:17Z
dc.date.issued2014
dc.description.abstractPurpose: Tissue oxygen (O-2) levels are among the most important and most quantifiable stimuli to which cells and tissues respond through inducible signaling pathways. Tumor O-2 levels are major determinants of the response to cancer therapy. Developing more accurate measurements and images of tissue O-2 partial pressure (pO(2)), assumes enormous practical, biological, and medical importance. Methods: We present a fundamentally new technique to image pO(2) in tumors and tissues with pulse electron paramagnetic resonance (EPR) imaging enabled by an injected, nontoxic, triaryl methyl (trityl) spin probe whose unpaired electron's slow relaxation rates report the tissue pO(2). Heretofore, virtually all in vivo EPR O-2 imaging measures pO(2) with the transverse electron spin relaxation rate, R-2e, which is susceptible to the self-relaxation confounding O-2 sensitivity. Results: We found that the trityl electron longitudinal relaxation rate, R-1e, is an order of magnitude less sensitive to confounding self-relaxation. R-1e imaging has greater accuracy and brings EPR O-2 images to an absolute pO(2) image, within uncertainties. Conclusion: R-1e imaging more accurately determines oxygenation of cancer and normal tissue in animal models than has been available. It will enable enhanced, rapid, noninvasive O-2 images for understanding oxygen biology and the relationship of oxygenation patterns to therapy outcome in living animal systems. (C) 2013 Wiley Periodicals, Inc.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationEpel, B., Bowman, M. K., Mailer, C., & Halpern, H. J. (2013). Absolute oxygen R1eimaging in vivo with pulse electron paramagnetic resonance. In Magnetic Resonance in Medicine (Vol. 72, Issue 2, pp. 362–368). Wiley. https://doi.org/10.1002/mrm.24926
dc.identifier.doi10.1002/mrm.24926
dc.identifier.orcidhttps://orcid.org/0000-0003-3464-9409
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12122
dc.languageEnglish
dc.language.isoen_US
dc.publisherWiley-Blackwell
dc.subjectoxygen
dc.subjectimaging
dc.subjectEPR
dc.subjectpulse
dc.subjectR-1
dc.subjectspin lattice relaxation
dc.subjectin vivo
dc.subjecttumor
dc.subjectTUMOR HYPOXIA
dc.subject250 MHZ
dc.subjectTRIARYLMETHYL RADICALS
dc.subjectSPIN-ECHO
dc.subjectBOLD MRI
dc.subjectIMAGES
dc.subjectRELAXATION
dc.subjectMEMBRANES
dc.subjectO-2
dc.subjectRadiology, Nuclear Medicine & Medical Imaging
dc.titleAbsolute Oxygen R-1e Imaging In Vivo with Pulse Electron Paramagnetic Resonanceen_US
dc.typeArticle
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