UA cloudflare authentication

 

Genome Size Changes by Duplication, Divergence, and Insertion in Caenorhabditis Worms

Simple item page
dc.contributor.authorAdams, Paula E.
dc.contributor.authorEggers, Victoria K.
dc.contributor.authorMillwood, Joshua D.
dc.contributor.authorSutton, John M.
dc.contributor.authorPienaar, Jason
dc.contributor.authorFierst, Janna L.
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherAuburn University
dc.contributor.otherFlorida International University
dc.date.accessioned2023-09-28T19:15:30Z
dc.date.available2023-09-28T19:15:30Z
dc.date.issued2023
dc.description.abstractGenome size has been measurable since the 1940s but we still do not understand genome size variation. Caenorhabditis nematodes show strong conservation of chromosome number but vary in genome size between closely related species. Androdioecy, where populations are composed of males and self-fertile hermaphrodites, evolved from outcrossing, female-male dioecy, three times in this group. In Caenorhabditis, androdioecious genomes are 10-30% smaller than dioecious species, but in the nematode Pristionchus, androdioecy evolved six times and does not correlate with genome size. Previous hypotheses include genome size evolution through: 1) Deletions and "genome shrinkage" in androdioecious species; 2) Transposable element (TE) expansion and DNA loss through large deletions (the "accordion model"); and 3) Differing TE dynamics in androdioecious and dioecious species. We analyzed nematode genomes and found no evidence for these hypotheses. Instead, nematode genome sizes had strong phylogenetic inertia with increases in a few dioecious species, contradicting the "genome shrinkage" hypothesis. TEs did not explain genome size variation with the exception of the DNA transposon Mutator which was twice as abundant in dioecious genomes. Across short and long evolutionary distances Caenorhabditis genomes evolved through small structural mutations including gene-associated duplications and insertions. Seventy-one protein families had significant, parallel decreases across androdioecious Caenorhabditis including genes involved in the sensory system, regulatory proteins and membrane-associated immune responses. Our results suggest that within a dynamic landscape of frequent small rearrangements in Caenorhabditis, reproductive mode mediates genome evolution by altering the precise fates of individual genes, proteins, and the phenotypes they underlie.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationAdams, P. E., Eggers, V. K., Millwood, J. D., Sutton, J. M., Pienaar, J., & Fierst, J. L. (2023). Genome Size Changes by Duplication, Divergence, and Insertion inCaenorhabditisWorms. In I. Arkhipova (Ed.), Molecular Biology and Evolution (Vol. 40, Issue 3). Oxford University Press (OUP). https://doi.org/10.1093/molbev/msad039
dc.identifier.doi10.1093/molbev/msad039
dc.identifier.orcidhttps://orcid.org/0000-0002-3639-8829
dc.identifier.orcidhttps://orcid.org/0000-0001-8477-4203
dc.identifier.orcidhttps://orcid.org/0000-0002-9631-392X
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11188
dc.languageEnglish
dc.language.isoen_US
dc.publisherOxford University Press
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectstructural variants
dc.subjectmutation
dc.subjectreproductive transitions
dc.subjectmolecular divergence
dc.subjectTRANSPOSABLE ELEMENTS
dc.subjectEVOLUTION
dc.subjectGENE
dc.subjectTOOL
dc.subjectDNA
dc.subjectFINDER
dc.subjectPOPULATIONS
dc.subjectEXPRESSION
dc.subjectMUTATIONS
dc.subjectRESOURCE
dc.subjectBiochemistry & Molecular Biology
dc.subjectEvolutionary Biology
dc.subjectGenetics & Heredity
dc.titleGenome Size Changes by Duplication, Divergence, and Insertion in Caenorhabditis Wormsen_US
dc.typeArticle
dc.typetext

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PMC10015627-msad039.pdf
Size:
1.4 MB
Format:
Adobe Portable Document Format
Download

Collections

Research and Publications - Department of Chemistry & Biochemistry