Mitochondrial Haplotypes Influence Metabolic Traits in Porcine Transmitochondrial Cybrids

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dc.contributor.authorYu, Guanghui
dc.contributor.authorXiang, Hai
dc.contributor.authorTian, Jianhui
dc.contributor.authorYin, Jingdong
dc.contributor.authorPinkert, Carl A.
dc.contributor.authorLi, Qiuyan
dc.contributor.authorZhao, Xingbo
dc.contributor.otherMinistry of Agriculture & Rural Affairs
dc.contributor.otherChina Agricultural University
dc.contributor.otherAuburn University
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-10-02T15:17:50Z
dc.date.available2023-10-02T15:17:50Z
dc.date.issued2015
dc.description.abstractIn farm animals, mitochondrial DNA mutations exist widely across breeds and individuals. In order to identify differences among mtDNA haplotypes, two porcine transmitochondrial cybrids were generated by fusion of a Lantang pig cell line devoid of mitochondrial DNA with enucleated cytoplasm from either a Large White pig or a Xiang pig harboring potentially divergent mitochondrial haplotypes. These cybrid cells were subjected to mitochondrial genome sequencing, copy number detecting and analysis of biochemical traits including succinate dehydrogenase (SDH) activity, ATP content and susceptibility to reactive oxygen species (ROS). The Lantang and Xiang mitochondrial genomes were highly homologous with only 18 polymorphic sites, and differed radically from the Large White with 201 and 198 mutations respectively. The Large White and Xiang cybrids exhibited similar mtDNA copy numbers and different values among biochemical traits, generated greater ROS production (P < 0.05) and less SDH activity (P < 0.05) and a lesser ATP content (P < 0.05). The results show that functional differences exist between cybrid cells which differ in mitochondrial genomic background. In conclusion, transmitochondrial cybrids provide the first direct evidence on pig biochemical traits linking different mitochondrial genome haplotypes.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationYu, G., Xiang, H., Tian, J., Yin, J., Pinkert, C. A., Li, Q., & Zhao, X. (2015). Mitochondrial Haplotypes Influence Metabolic Traits in Porcine Transmitochondrial Cybrids. In Scientific Reports (Vol. 5, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/srep13118
dc.identifier.doi10.1038/srep13118
dc.identifier.orcidhttps://orcid.org/0000-0001-8866-7931
dc.identifier.orcidhttps://orcid.org/0000-0001-7460-3881
dc.identifier.orcidhttps://orcid.org/0000-0003-4623-0568
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12603
dc.languageEnglish
dc.language.isoen_US
dc.publisherNature Portfolio
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCARCASS TRAITS
dc.subjectDOMESTIC PIG
dc.subjectDNA
dc.subjectMTDNA
dc.subjectPOLYMORPHISMS
dc.subjectPROTEIN
dc.subjectSUBSTITUTIONS
dc.subjectTRANSMISSION
dc.subjectASSOCIATION
dc.subjectMODELS
dc.subjectMultidisciplinary Sciences
dc.titleMitochondrial Haplotypes Influence Metabolic Traits in Porcine Transmitochondrial Cybridsen_US
dc.typeArticle
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