Reversible Dimerization of Human Serum Albumin

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dc.contributor.authorChubarov, Alexey
dc.contributor.authorSpitsyna, Anna
dc.contributor.authorKrumkacheva, Olesya
dc.contributor.authorMitin, Dmitry
dc.contributor.authorSuvorov, Daniil
dc.contributor.authorTormyshev, Victor
dc.contributor.authorFedin, Matvey
dc.contributor.authorBowman, Michael K.
dc.contributor.authorBagryanskaya, Elena
dc.contributor.otherInstitute of Chemical Biology & Fundamental Medicine, Siberian Branch of the RAS
dc.contributor.otherRussian Academy of Sciences
dc.contributor.otherNovosibirsk State University
dc.contributor.otherVorozhtsov Novosibirsk Institute of Organic Chemistry
dc.contributor.otherInternational Tomography Center, Siberian Branch of Russian Academy of Sciences
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2021-08-25T21:04:16Z
dc.date.available2021-08-25T21:04:16Z
dc.date.issued2020
dc.description.abstractPulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to detect and characterize reversible non-covalent dimers of Human Serum Albumin (HSA), the most abundant protein in human plasma. The spin labels, MTSL and OX063, were attached to Cys-34 and these chemical modifications of Cys-34 did affect the dimerization of HSA, indicating that other post-translational modifications can modulate dimer formation. At physiologically relevant concentrations, HSA does form weak, non-covalent dimers with a well-defined structure. Dimer formation is readily reversible into monomers. Dimerization is very relevant to the role of HSA in the transport, binding, and other physiological processes.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.citationChubarov, A., Spitsyna, A., Krumkacheva, O., Mitin, D., Suvorov, D., Tormyshev, V., Fedin, M., Bowman, M., Bagryanskaya, E. (2020): Reversible Dimerization of Human Serum Albumin. Molecules. 26(1).
dc.identifier.doi10.3390/molecules26010108
dc.identifier.orcidhttps://orcid.org/0000-0002-0777-5103
dc.identifier.orcidhttps://orcid.org/0000-0002-0537-5755
dc.identifier.orcidhttps://orcid.org/0000-0002-5708-7595
dc.identifier.orcidhttps://orcid.org/0000-0003-0057-383X
dc.identifier.orcidhttps://orcid.org/0000-0003-0454-979X
dc.identifier.urihttp://ir.ua.edu/handle/123456789/8069
dc.languageEnglish
dc.language.isoen_US
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjecthuman serum albumin
dc.subjectpulse dipole EPR
dc.subjectaggregation
dc.subjectDIMER
dc.subjectBINDING
dc.subjectDYNAMICS
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry
dc.titleReversible Dimerization of Human Serum Albuminen_US
dc.typetext
dc.typeArticle

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