Secretome identification of immune cell factors mediating metastatic cell homing

Simple item page
dc.contributor.authorAguado, Brian A.
dc.contributor.authorWu, Jia J.
dc.contributor.authorAzarin, Samira M.
dc.contributor.authorNanavati, Dhaval
dc.contributor.authorRao, Shreyas S.
dc.contributor.authorBushnell, Grace G.
dc.contributor.authorMedicherla, Chaitanya B.
dc.contributor.authorShea, Lonnie D.
dc.contributor.otherNorthwestern University
dc.contributor.otherUniversity of Minnesota Twin Cities
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherUniversity of Michigan
dc.contributor.otherFeinberg School of Medicine
dc.date.accessioned2023-09-28T19:11:46Z
dc.date.available2023-09-28T19:11:46Z
dc.date.issued2015
dc.description.abstractMetastatic cell homing is a complex process mediated in part by diffusible factors secreted from immune cells found at a pre-metastatic niche. We report on connecting secretomics and TRanscriptional Activity CEll aRray (TRACER) data to identify functional paracrine interactions between immune cells and metastatic cells as novel mediators of homing. Metastatic breast cancer mouse models were used to generate a diseased splenocyte conditioned media (D-SCM) containing immune cell secreted factors. MDA-MB-231 metastatic cell activity including cell invasion, migration, transendothelial migration, and proliferation were increased in D-SCM relative to control media. Our D-SCM secretome analysis yielded 144 secreted factor candidates that contribute to increased metastatic cell activity. The functional mediators of homing were identified using MetaCore software to determine interactions between the immune cell secretome and the TRACER-identified active transcription factors within metastatic cells. Among the 5 candidate homing factors identified, haptoglobin was selected and validated in vitro and in vivo as a key mediator of homing. Our studies demonstrate a novel systems biology approach to identify functional signaling factors associated with a cellular phenotype, which provides an enabling tool that complements large-scale protein identification provided by proteomics.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationAguado, B. A., Wu, J. J., Azarin, S. M., Nanavati, D., Rao, S. S., Bushnell, G. G., Medicherla, C. B., & Shea, L. D. (2015). Secretome identification of immune cell factors mediating metastatic cell homing. In Scientific Reports (Vol. 5, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/srep17566
dc.identifier.doi10.1038/srep17566
dc.identifier.orcidhttps://orcid.org/0000-0002-8891-803X
dc.identifier.orcidhttps://orcid.org/0000-0001-7649-0171
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11029
dc.languageEnglish
dc.language.isoen_US
dc.publisherNature Portfolio
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBREAST-CANCER CELLS
dc.subjectIN-VIVO
dc.subjectHAPTOGLOBIN
dc.subjectPROTEOMICS
dc.subjectRECRUITMENT
dc.subjectMICROARRAY
dc.subjectEXPRESSION
dc.subjectDISCOVERY
dc.subjectMIGRATION
dc.subjectPROTEIN
dc.subjectMultidisciplinary Sciences
dc.titleSecretome identification of immune cell factors mediating metastatic cell homingen_US
dc.typeArticle
dc.typetext

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PMC4669442-srep17566.pdf
Size:
1.97 MB
Format:
Adobe Portable Document Format
Download

Collections

Research and Publications - Department of Chemical & Biological Engineering