Genetic characterization of caffeine degradation by bacteria and its potential applications

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dc.contributor.authorSummers, Ryan M.
dc.contributor.authorMohanty, Sujit K.
dc.contributor.authorGopishetty, Sridhar
dc.contributor.authorSubramanian, Mani
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherUniversity of Iowa
dc.date.accessioned2023-09-28T19:11:48Z
dc.date.available2023-09-28T19:11:48Z
dc.date.issued2015
dc.description.abstractThe ability of bacteria to grow on caffeine as sole carbon and nitrogen source has been known for over 40 years. Extensive research into this subject has revealed two distinct pathways, N-demethylation and C-8 oxidation, for bacterial caffeine degradation. However, the enzymological and genetic basis for bacterial caffeine degradation has only recently been discovered. This review article discusses the recent discoveries of the genes responsible for both N-demethylation and C-8 oxidation. All of the genes for the N-demethylation pathway, encoding enzymes in the Rieske oxygenase family, reside on 13.2-kb genomic DNA fragment found in Pseudomonas putidaCBB5. A nearly identical DNA fragment, with homologous genes in similar orientation, is found in Pseudomonas sp. CES. Similarly, genes for C-8 oxidation of caffeine have been located on a 25.2-kb genomic DNA fragment of Pseudomonas sp. CBB1. The C-8 oxidation genes encode enzymes similar to those found in the uric acid metabolic pathway of Klebsiella pneumoniae. Various biotechnological applications of these genes responsible for bacterial caffeine degradation, including bio-decaffeination, remediation of caffeine-contaminated environments, production of chemical and fuels and development of diagnostic tests have also been demonstrated.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationSummers, R. M., Mohanty, S. K., Gopishetty, S., & Subramanian, M. (2015). Genetic characterization of caffeine degradation by bacteria and its potential applications. In Microbial Biotechnology (Vol. 8, Issue 3, pp. 369–378). Wiley. https://doi.org/10.1111/1751-7915.12262
dc.identifier.doi10.1111/1751-7915.12262
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11032
dc.languageEnglish
dc.language.isoen_US
dc.publisherWiley
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPSEUDOMONAS-PUTIDA CBB5
dc.subjectSP STRAIN CBB1
dc.subjectMIXED CULTURE CONSORTIUM
dc.subjectURIC-ACID
dc.subjectKLEBSIELLA-PNEUMONIAE
dc.subjectPURINE ALKALOIDS
dc.subjectMICROBIAL-PRODUCTION
dc.subjectTRIMETHYLURIC ACID
dc.subjectFILAMENTOUS FUNGI
dc.subjectMETABOLISM
dc.subjectBiotechnology & Applied Microbiology
dc.subjectMicrobiology
dc.titleGenetic characterization of caffeine degradation by bacteria and its potential applicationsen_US
dc.typeReview
dc.typetext

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