Reprogramming of fibroblast cells to totipotent state by DNA demethylation

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dc.contributor.authorGhazimoradi, Mohammad H.
dc.contributor.authorHasegawa, Kouichi
dc.contributor.authorZolghadr, Ehsan
dc.contributor.authorMontazeri, Samaneh
dc.contributor.authorFarivar, Shirin
dc.contributor.otherShahid Beheshti University
dc.contributor.otherKyoto University
dc.contributor.otherDepartment of Biotechnology (DBT) India
dc.contributor.otherInstitute for Stem Cell Biology & Regenerative Medicine - inStem
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherAcademic Center for Education, Culture & Research (ACECR)
dc.date.accessioned2023-09-28T20:18:14Z
dc.date.available2023-09-28T20:18:14Z
dc.date.issued2023
dc.description.abstractMany attempts have been made to induce high-quality embryonic stem cells such as pluripotent stem cells and totipotent stem cells, but challenges remain to be overcome such as appropriate methods and sources. Demethylation of the genome after fertilization is an important step to initiate zygote gene activation, which can lead to the development of new embryos. Here, we tried to induce totipotent stem cells by mimicking DNA demethylation patterns of the embryo. Our data showed, after induction of DNA demethylation via chemicals or knockdown of Dnmts, cells positive for Nanog, and Cdx2 emerged. These cells could differentiate into the pluripotent and trophoblast lineage cells in-vitro. After transferring these cells to the uterus, they can implant and form embryo-like structures. Our study showed the importance of DNA demethylation roles in totipotent stem cell induction and a new and easy way to induce this cell type.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationGhazimoradi, M. H., Hasegawa, K., Zolghadr, E., Montazeri, S., & Farivar, S. (2023). Reprogramming of fibroblast cells to totipotent state by DNA demethylation. In Scientific Reports (Vol. 13, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/s41598-023-28457-8
dc.identifier.doi10.1038/s41598-023-28457-8
dc.identifier.orcidhttps://orcid.org/0000-0002-5957-5955
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11721
dc.languageEnglish
dc.language.isoen_US
dc.publisherNature Portfolio
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMETHYLATION
dc.subjectTRANSDIFFERENTIATION
dc.subjectDIFFERENTIATION
dc.subjectDYNAMICS
dc.subjectMOUSE
dc.subjectMultidisciplinary Sciences
dc.titleReprogramming of fibroblast cells to totipotent state by DNA demethylationen_US
dc.typeArticle
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