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Disruption of dopamine homeostasis has sexually dimorphic effects on senescence characteristics of Drosophila melanogaster

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dc.contributor.authorBednarova, Andrea
dc.contributor.authorHanna, Marley E.
dc.contributor.authorRakshit, Kuntol
dc.contributor.authorO'Donnell, Janis M.
dc.contributor.authorKrishnan, Natraj
dc.contributor.otherMississippi State University
dc.contributor.otherCzech Academy of Sciences
dc.contributor.otherBiology Centre of the Czech Academy of Sciences
dc.contributor.otherMayo Clinic
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherArkansas State University
dc.date.accessioned2023-09-28T22:03:58Z
dc.date.available2023-09-28T22:03:58Z
dc.date.issued2017
dc.description.abstractThe neurotransmitter dopamine (DA) is known to be involved in a multitude of physiological processes. We investigated sexually dimorphic effects of disruptions in DA homeostasis and its relationship to senescence using three different Drosophila melanogaster mutants namely Catsup (Catsup(26)) with elevated DA levels, and pale (ple(2)), Punch (Pu-Z22) with depleted DA levels. In all genotypes including controls, DA levels were significantly lower in old (45-50-day-old) flies compared with young (3-5-day-old) in both sexes. Interestingly, females had lower DA content than males at young age whereas this difference was not observed in old age, suggesting that males had a larger decline in DA levels with age. Females, in general, were longer lived compared with males in all genotypes except ple(2) mutants with depleted DA levels. This phenotype was abolished in the ple(2) rescue flies. Interestingly, females also demonstrated marked age-related decline in circadian locomotor activity compared with males. Old Catsup 26 males with elevated DA levels accumulated significantly lower levels of lipid peroxidation product 4-hydroxy 2-nonenal (4-HNE) compared with age-matched wild type, ple(2) and Pu-Z22 mutant males. In Catsup 26 revertant lines this phenomenon was absent. We also observed a sexually dimorphic response in the expression levels of key stress and aging associated and/or related transcription factor genes across genotypes with elevated or depleted DA levels which was reverted to wild type levels in specific rescue lines. Taken together, our results reveal a novel sexually dimorphic involvement of DA in senescence characteristics of D. melanogaster.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationBednářová, A., Hanna, M. E., Rakshit, K., O’Donnell, J. M., & Krishnan, N. (2017). Disruption of dopamine homeostasis has sexually dimorphic effects on senescence characteristics ofDrosophila melanogaster. In C. G. Galizia (Ed.), European Journal of Neuroscience (Vol. 45, Issue 6, pp. 816–825). Wiley. https://doi.org/10.1111/ejn.13525
dc.identifier.doi10.1111/ejn.13525
dc.identifier.orcidhttps://orcid.org/0000-0001-6832-259X
dc.identifier.orcidhttps://orcid.org/0000-0002-6822-6351
dc.identifier.orcidhttps://orcid.org/0000-0002-6822-6351
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12241
dc.languageEnglish
dc.language.isoen_US
dc.publisherWiley
dc.subject4-hydroxynonenal
dc.subjectaging
dc.subjectcircadian rhythms
dc.subjectlifespan
dc.subjectprotein carbonyl
dc.subjectOXIDATIVE STRESS
dc.subjectTYROSINE-HYDROXYLASE
dc.subjectLOCOMOTOR-ACTIVITY
dc.subjectJUVENILE-HORMONE
dc.subjectGENE-EXPRESSION
dc.subjectPROTEIN
dc.subjectAMPK
dc.subjectSYSTEM
dc.subjectTISSUE
dc.subject20-HYDROXYECDYSONE
dc.subjectNeurosciences
dc.titleDisruption of dopamine homeostasis has sexually dimorphic effects on senescence characteristics of Drosophila melanogasteren_US
dc.typeArticle
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