Drosophila Pupal Abdomen Immunohistochemistry

dc.contributor.authorWang, Wei
dc.contributor.authorYoder, John H.
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-10-02T15:14:39Z
dc.date.available2023-10-02T15:14:39Z
dc.date.issued2011
dc.description.abstractThe Drosophila pupal abdomen is an established model system for the study of epithelial morphogenesis and the development of sexually dimorphic morphologies(1-3). During pupation, which spans approximately 96 hours (at 25 degrees C), proliferating populations of imaginal cells replace the larval epidermis to generate the adult abdominal segments. These imaginal cells, born during embryogenesis, exist as lateral pairs of histoblast nests in each abdominal segment of the larvae. Four pairs of histoblast nests give rise to the adult dorsal cuticle (anterior and posterior dorsal nests), the ventral cuticle (ventral nests) and the spiracles associated with each segment (spiracle nests)(4). Upon puparation, these diploid cells (distinguishable by size from the larger polyploid larval epidermal cells-LECs) begin a stereotypical process of proliferation, migration and replacement of the LECs. Various molecular and genetic tools can be employed to investigate the contributions of genetic pathways involved in morphogenesis of the adult abdomen. Ultimate adult phenotypes are typically analyzed following dissection of adult abdominal cuticles. However, investigation of the underlying molecular processes requires immunohistochemical analyses of the pupal epithelium, which present unique challenges. Temporally dynamic morphogenesis and the interactions of two distinct epithelial populations (larval and imaginal) generate a fragile tissue prone to excessive cell loss during dissection and subsequent processing. We have developed methods of dissection, fixation, mounting and imaging of the Drosophila pupal abdominem epithelium for immunohistochemical studies that generate consistent high quality samples suitable for confocal or standard fluorescent microscopy.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationWang, W., & Yoder, J. H. (2011). <em>Drosophila</em> Pupal Abdomen Immunohistochemistry. In Journal of Visualized Experiments (Issue 56). MyJove Corporation. https://doi.org/10.3791/3139
dc.identifier.doi10.3791/3139
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12463
dc.languageEnglish
dc.language.isoen_US
dc.publisherMyJove Corporation
dc.rights.licenseAttribution-NoDerivatives 4.0 International (CC BY-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectImmunology
dc.subjectIssue 56
dc.subjectDrosophila
dc.subjectimmunohistochemistry
dc.subjectpupae
dc.subjectabdomen
dc.subjectepithelium
dc.subjectantibody
dc.subjectMultidisciplinary Sciences
dc.titleDrosophila Pupal Abdomen Immunohistochemistryen_US
dc.typeArticle
dc.typetext
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
10.37913139.pdf
Size:
203.62 KB
Format:
Adobe Portable Document Format