ObjectiveThe purpose of this study was to determine the feasibility of using mobile standing desks to address work-related sedentary behavior for adults working in flexible positions.MethodsUniversity employees (N = 20) in flexible positions used a mobile standing desk for 8 weeks and completed one focus group. Paired t tests were used to determine differences in workplace behavior. Focus group transcripts were analyzed using thematic analysis.ResultsWork-related sitting decreased (P = 0.010, -45.88 minutes) and standing increased (P = 0.016, +41.28 minutes). Advantages included mobility, enhanced attention to tasks, and ease of use. Disadvantages included the device's design, built environment barriers, and impact of work/life circumstances.ConclusionsThe mobile standing desk resulted in a decrease in sitting consistent with traditional standing desk-based interventions. Future interventions should consider including goal setting, reminders, feedback, and incentives.