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Comparative proteomic analysis of three Chinese hamster ovary (CHO) host cells

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dc.contributor.authorXu, Ningning
dc.contributor.authorMa, Chao
dc.contributor.authorOu, Jianfa
dc.contributor.authorSun, Wanqi Wendy
dc.contributor.authorZhou, Lufang
dc.contributor.authorHu, Hui
dc.contributor.authorLiu, Xiaoguang Margaret
dc.contributor.otherUniversity of Alabama Birmingham
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:11:28Z
dc.date.available2023-09-28T19:11:28Z
dc.date.issued2017
dc.description.abstractChinese hamster ovary (CHO)1 cells have been widely used to express heterologous genes and produce therapeutic proteins in biopharmaceutical industry. Different CHO host cells have distinct cell growth rates and protein expression characteristics. In this study, the expression of about 1307 host proteins in three sublines, i.e. CHO K1, CHO S and CHO/dihydrofolate reductase (dhfr)(-), were investigated and compared using proteomic analysis. The proteins involved in cell growth, glycolysis, tricarboxylic acid cycle, transcription, translation and glycosylation were quantitated using Liquid chromatography tandem-mass spectrometry (LC-MS/MS). The key host cell proteins that regulate the kinetics of cell growth and the magnitude of protein expression levels were identified. Furthermore, several rational cell engineering strategies on how to combine the desired features of fast cell growth and efficient production of therapeutic proteins into one new super CHO host cell have been proposed. (C) 2017 Elsevier B.V. All rights reserved.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationXu, N., Ma, C., Ou, J., Sun, W. W., Zhou, L., Hu, H., & Liu, X. M. (2017). Comparative proteomic analysis of three Chinese hamster ovary (CHO) host cells. In Biochemical Engineering Journal (Vol. 124, pp. 122–129). Elsevier BV. https://doi.org/10.1016/j.bej.2017.05.007
dc.identifier.doi10.1016/j.bej.2017.05.007
dc.identifier.orcidhttps://orcid.org/0000-0002-1321-8442
dc.identifier.orcidhttps://orcid.org/0000-0002-4617-9750
dc.identifier.urihttps://ir.ua.edu/handle/123456789/10989
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier
dc.subjectCHO sublines
dc.subjectComparative proteomics
dc.subjectCell growth
dc.subjectProtein expression
dc.subjectRegulators
dc.subjectZINC-FINGER NUCLEASES
dc.subjectMAMMALIAN-CELLS
dc.subjectGENE KNOCKOUT
dc.subjectENDOPLASMIC-RETICULUM
dc.subjectEXPRESSION
dc.subjectLINE
dc.subjectPRODUCTIVITY
dc.subjectCULTURE
dc.subjectOVEREXPRESSION
dc.subjectIDENTIFICATION
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering, Chemical
dc.titleComparative proteomic analysis of three Chinese hamster ovary (CHO) host cellsen_US
dc.typeArticle
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