Differentially Expressed Forms of 1-L-myo-Inositol-1-Phosphate Synthase (EC 5.5.1.4) in Phaseolus vulgaris
| dc.contributor.author | Johnson, Margaret Dean | |
| dc.contributor.author | Wang, Xiaohong | |
| dc.date.accessioned | 2026-04-01T17:53:24Z | |
| dc.date.available | 2026-04-01T17:53:24Z | |
| dc.date.copyright | 1996 | |
| dc.date.issued | 1996-07-19 | |
| dc.description | Open Access Article | |
| dc.description.abstract | We have characterized two distinct polypeptides with 1-L-myo-inositol-1-phosphate synthase (MI-1-P synthase) activity that are differentially expressed during development in Phaseolus vulgaris. Western analyses, enzyme assays, and partial purification of MI-1-P synthase during embryonic and postembryonic development show that its expression is temporally and spatially regulated. Developmental Western analyses of soluble proteins detect a small protein, approximately 33 kDa, with MI-1-P synthase activity during the globular stage (stage II) of embryogenesis and in mature roots. Expression of this small protein is also enriched in thylakoidal membranes of fractionated leaf chloroplasts, although Western analyses of total soluble leaf proteins show no cross-reacting material. In contrast, a larger protein, approximately 56 kDa, with MI-1-P synthase activity is present during the cotyledonary phase (stage IV) of embryogenesis in green cotyledons of seedlings and in young roots. | |
| dc.description.sponsorship | This work was supported in part by National Science Foundation Grant MCB-9307092. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. | |
| dc.description.version | Version of record | |
| dc.format.extent | 4 p. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Johnson, M. D. & Wang, X. (1996). Differentially expressed forms of 1-L-myo-Inositol-1-Phosphate Synthase (EC) in Phaseolus vulgaris*. Journal of Biological Chemistry, 271(29), 17215-17218. https://doi.org/10.1074/jbc.271.29.17215 | |
| dc.identifier.doi | 10.1074/jbc.271.29.17215 | |
| dc.identifier.uri | https://ir.ua.edu/handle/123456789/17731 | |
| dc.language | English | |
| dc.language.iso | en_US | |
| dc.publisher | American Society for Biochemistry and Molecular Biology | |
| dc.rights.holder | ASBMB | |
| dc.rights.license | CC BY 4.0 | |
| dc.title | Differentially Expressed Forms of 1-L-myo-Inositol-1-Phosphate Synthase (EC 5.5.1.4) in Phaseolus vulgaris | |
| dc.type | Article | |
| dcterms.license | This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You are not required to obtain permission to reuse this article. | |
| publicationissue.issueNumber | 29 | |
| publicationvolume.volumeNumber | 271 |