dtorsin, the Drosophila Ortholog of the Early-Onset Dystonia TOR1A (DYT1), Plays a Novel Role in Dopamine Metabolism

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dc.contributor.authorWakabayashi-Ito, Noriko
dc.contributor.authorDoherty, Olugbenga M.
dc.contributor.authorMoriyama, Hideaki
dc.contributor.authorBreakefield, Xandra O.
dc.contributor.authorGusella, James F.
dc.contributor.authorO'Donnell, Janis M.
dc.contributor.authorIto, Naoto
dc.contributor.otherHarvard University
dc.contributor.otherMassachusetts General Hospital
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.contributor.otherUniversity of Nebraska Lincoln
dc.contributor.otherHarvard Medical School
dc.date.accessioned2023-10-02T15:14:28Z
dc.date.available2023-10-02T15:14:28Z
dc.date.issued2011
dc.description.abstractDystonia represents the third most common movement disorder in humans. At least 15 genetic loci (DYT1-15) have been identified and some of these genes have been cloned. TOR1A (formally DYT1), the gene responsible for the most common primary hereditary dystonia, encodes torsinA, an AAA ATPase family protein. However, the function of torsinA has yet to be fully understood. Here, we have generated and characterized a complete loss-of-function mutant for dtorsin, the only Drosophila ortholog of TOR1A. Null mutation of the X-linked dtorsin was semi-lethal with most male flies dying by the pre-pupal stage and the few surviving adults being sterile and slow moving, with reduced cuticle pigmentation and thin, short bristles. Third instar male larvae exhibited locomotion defects that were rescued by feeding dopamine. Moreover, biochemical analysis revealed that the brains of third instar larvae and adults heterozygous for the loss-of-function dtorsin mutation had significantly reduced dopamine levels. The dtorsin mutant showed a very strong genetic interaction with Pu (Punch: GTP cyclohydrolase), the ortholog of the human gene underlying DYT14 dystonia. Biochemical analyses revealed a severe reduction of GTP cyclohydrolase protein and activity, suggesting that dtorsin plays a novel role in dopamine metabolism as a positive-regulator of GTP cyclohydrolase protein. This dtorsin mutant line will be valuable for understanding this relationship and potentially other novel torsin functions that could play a role in human dystonia.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationWakabayashi-Ito, N., Doherty, O. M., Moriyama, H., Breakefield, X. O., Gusella, J. F., O’Donnell, J. M., & Ito, N. (2011). dtorsin, the Drosophila Ortholog of the Early-Onset Dystonia TOR1A (DYT1), Plays a Novel Role in Dopamine Metabolism. In B. D. McCabe (Ed.), PLoS ONE (Vol. 6, Issue 10, p. e26183). Public Library of Science (PLoS). https://doi.org/10.1371/journal.pone.0026183
dc.identifier.doi10.1371/journal.pone.0026183
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12446
dc.languageEnglish
dc.language.isoen_US
dc.publisherPLOS
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectTYROSINE-HYDROXYLASE
dc.subjectTORSION DYSTONIA
dc.subjectPHENOTYPIC ANALYSIS
dc.subjectPROTEIN TORSINA
dc.subjectPUNCH LOCUS
dc.subjectMOUSE MODEL
dc.subjectGENE
dc.subjectIDENTIFICATION
dc.subjectLOCOMOTION
dc.subjectBIOSYNTHESIS
dc.subjectMultidisciplinary Sciences
dc.titledtorsin, the Drosophila Ortholog of the Early-Onset Dystonia TOR1A (DYT1), Plays a Novel Role in Dopamine Metabolismen_US
dc.typeArticle
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