Endoplasmic reticulum stress impairment in the spinal dorsal horn of a neuropathic pain model

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dc.contributor.authorZhang, Enji
dc.contributor.authorYi, Min-Hee
dc.contributor.authorShin, Nara
dc.contributor.authorBaek, Hyunjung
dc.contributor.authorKim, Sena
dc.contributor.authorKim, Eunjee
dc.contributor.authorKwon, Kisang
dc.contributor.authorLee, Sunyeul
dc.contributor.authorKim, Hyun-Woo
dc.contributor.authorBae, Yong Chul
dc.contributor.authorKim, Yonghyun
dc.contributor.authorKwon, O. -Yu
dc.contributor.authorLee, Won Hyung
dc.contributor.authorKim, Dong Woon
dc.contributor.otherChungnam National University
dc.contributor.otherChungnam National University Hospital
dc.contributor.otherKyungpook National University
dc.contributor.otherYanbian University
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:11:48Z
dc.date.available2023-09-28T19:11:48Z
dc.date.issued2015
dc.description.abstractEndoplasmic reticulum (ER) stress has been implicated in neurodegenerative diseases, but its role in neuropathic pain remains unclear. In this study, we examined the ER stress and the unfolded protein response (UPR) activation in a L5 spinal nerve ligation (SNL)-induced rat neuropathic pain model. SNL-induced neuropathic pain was assessed behaviorally using the CatWalk system, and histologically with microglial activation in the dorsal spinal horn. L5 SNL induced BIP upregulation in the neuron of superficial laminae of dorsal spinal horn. It also increased the level of ATF6 and intracellular localization into the nuclei in the neurons. Moreover, spliced XBP1 was also markedly elevated in the ipsilateral spinal dorsal horn. The PERK-elF2 pathway was activated in astrocytes of the spinal dorsal horn in the SNL model. In addition, electron microscopy revealed the presence of swollen cisternae in the dorsal spinal cord after SNL. Additionally, inhibition of the ATF6 pathway by intrathecal treatment with ATF6 siRNA reduced pain behaviors and BIP expression in the dorsal horn. The results suggest that ER stress might be involved in the induction and maintenance of neuropathic pain. Furthermore, a disturbance in UPR signaling may render the spinal neurons vulnerable to peripheral nerve injury or neuropathic pain stimuli.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationZhang, E., Yi, M.-H., Shin, N., Baek, H., Kim, S., Kim, E., Kwon, K., Lee, S., Kim, H.-W., Chul Bae, Y., Kim, Y., Kwon, O.-Y., Lee, W. H., & Kim, D. W. (2015). Endoplasmic reticulum stress impairment in the spinal dorsal horn of a neuropathic pain model. In Scientific Reports (Vol. 5, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1038/srep11555
dc.identifier.doi10.1038/srep11555
dc.identifier.orcidhttps://orcid.org/0000-0001-7691-1394
dc.identifier.orcidhttps://orcid.org/0000-0001-6344-1258
dc.identifier.orcidhttps://orcid.org/0000-0002-8796-8967
dc.identifier.orcidhttps://orcid.org/0000-0002-6124-5138
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11031
dc.languageEnglish
dc.language.isoen_US
dc.publisherNature Portfolio
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectUNFOLDED PROTEIN RESPONSE
dc.subjectPERIPHERAL-NERVE INJURY
dc.subjectCELL-SURVIVAL
dc.subjectMESSENGER-RNA
dc.subjectER STRESS
dc.subjectAUTOPHAGY
dc.subjectDISEASE
dc.subjectPROTEOLYSIS
dc.subjectACTIVATION
dc.subjectINDUCTION
dc.subjectMultidisciplinary Sciences
dc.titleEndoplasmic reticulum stress impairment in the spinal dorsal horn of a neuropathic pain modelen_US
dc.typeArticle
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