Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos

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dc.contributor.authorJenny, Matthew J.
dc.contributor.authorAluru, Neelakanteswar
dc.contributor.authorHahn, Mark E.
dc.contributor.otherWoods Hole Oceanographic Institution
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-10-02T15:14:23Z
dc.date.available2023-10-02T15:14:23Z
dc.date.issued2012
dc.description.abstractAlthough many drugs and environmental chemicals are teratogenic, the mechanisms by which most toxicants disrupt embryonic development are not well understood. MicroRNAs, single-stranded RNA molecules of similar to 22 nt that regulate protein expression by inhibiting mRNA translation and promoting mRNA sequestration or degradation, are important regulators of a variety of cellular processes including embryonic development and cellular differentiation. Recent studies have demonstrated that exposure to xenobiotics can alter microRNA expression and contribute to the mechanisms by which environmental chemicals disrupt embryonic development. In this study we tested the hypothesis that developmental exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a well-known teratogen, alters microRNA expression during zebrafish development. We exposed zebrafish embryos to DMSO (0.1%) or TCDD (5 nM) for 1 h at 30 hours post fertilization (hpf) and measured microRNA expression using several methods at 36 and 60 hpf. TCDD caused strong induction of CYP1A at 36 hpf (62-fold) and 60 hpf (135-fold) as determined by real-time RT-PCR, verifying the effectiveness of the exposure. MicroRNA expression profiles were determined using microarrays (Agilent and Exicion), next-generation sequencing (SOLiD), and real-time RT-PCR. The two microarray platforms yielded results that were similar but not identical; both showed significant changes in expression of miR-451, 23a, 23b, 24 and 27e at 60 hpf. Multiple analyses were performed on the SOLiD sequences yielding a total of 16 microRNAs as differentially expressed by TCDD in zebrafish embryos. However, miR-27e was the only microRNA to be identified as differentially expressed by all three methods (both microarrays, SOLiD sequencing, and real-time RT-PCR). These results suggest that TCDD exposure causes modest changes in expression of microRNAs, including some (miR-451, 23a, 23b, 24 and 27e) that are critical for hematopoiesis and cardiovascular development. (C) 2012 Elsevier Inc. All rights reserved.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationJenny, M. J., Aluru, N., & Hahn, M. E. (2012). Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos. In Toxicology and Applied Pharmacology (Vol. 264, Issue 2, pp. 262–273). Elsevier BV. https://doi.org/10.1016/j.taap.2012.08.007
dc.identifier.doi10.1016/j.taap.2012.08.007
dc.identifier.orcidhttps://orcid.org/0000-0003-4358-2082
dc.identifier.orcidhttps://orcid.org/0000-0002-1834-2629
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12437
dc.languageEnglish
dc.language.isoen_US
dc.publisherElsevier
dc.subjectDioxin
dc.subjectMicroRNA
dc.subjectZebra fish
dc.subjectDevelopment
dc.subjectRECEPTOR NUCLEAR TRANSLOCATOR
dc.subjectGENE-EXPRESSION
dc.subjectDIFFERENTIAL EXPRESSION
dc.subjectDEVELOPMENTAL TOXICITY
dc.subjectCARDIAC-HYPERTROPHY
dc.subjectDOWN-REGULATION
dc.subjectDANIO-RERIO
dc.subjectSMALL RNAS
dc.subjectTCDD
dc.subjectLIVER
dc.subjectPharmacology & Pharmacy
dc.subjectToxicology
dc.titleEffects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryosen_US
dc.typeArticle
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