Growth and stress response mechanisms underlying post- feeding regenerative organ growth in the Burmese python

dc.contributor.authorAndrew, Audra L.
dc.contributor.authorPerry, Blair W.
dc.contributor.authorCard, Daren C.
dc.contributor.authorSchield, Drew R.
dc.contributor.authorRuggiero, Robert P.
dc.contributor.authorMcGaugh, Suzanne E.
dc.contributor.authorChoudhary, Amit
dc.contributor.authorSecor, Stephen M.
dc.contributor.authorCastoe, Todd A.
dc.contributor.otherUniversity of Texas Arlington
dc.contributor.otherUniversity of Minnesota Twin Cities
dc.contributor.otherHarvard University
dc.contributor.otherMassachusetts Institute of Technology (MIT)
dc.contributor.otherBroad Institute
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:11:45Z
dc.date.available2023-09-28T19:11:45Z
dc.date.issued2017
dc.description.abstractBackground: Previous studies examining post-feeding organ regeneration in the Burmese python (Python molurus bivittatus) have identified thousands of genes that are significantly differentially regulated during this process. However, substantial gaps remain in our understanding of coherent mechanisms and specific growth pathways that underlie these rapid and extensive shifts in organ form and function. Here we addressed these gaps by comparing gene expression in the Burmese python heart, liver, kidney, and small intestine across pre- and post-feeding time points (fasted, one day post-feeding, and four days post-feeding), and by conducting detailed analyses of molecular pathways and predictions of upstream regulatory molecules across these organ systems. Results: Identified enriched canonical pathways and upstream regulators indicate that while downstream transcriptional responses are fairly tissue specific, a suite of core pathways and upstream regulator molecules are shared among responsive tissues. Pathways such as mTOR signaling, PPAR/LXR/RXR signaling, and NRF2-mediated oxidative stress response are significantly differentially regulated in multiple tissues, indicative of cell growth and proliferation along with coordinated cell-protective stress responses. Upstream regulatory molecule analyses identify multiple growth factors, kinase receptors, and transmembrane receptors, both within individual organs and across separate tissues. Downstream transcription factors MYC and SREBF are induced in all tissues. Conclusions: These results suggest that largely divergent patterns of post-feeding gene regulation across tissues are mediated by a core set of higher-level signaling molecules. Consistent enrichment of the NRF2-mediated oxidative stress response indicates this pathway may be particularly important in mediating cellular stress during such extreme regenerative growth.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationAndrew, A. L., Perry, B. W., Card, D. C., Schield, D. R., Ruggiero, R. P., McGaugh, S. E., Choudhary, A., Secor, S. M., & Castoe, T. A. (2017). Growth and stress response mechanisms underlying post-feeding regenerative organ growth in the Burmese python. In BMC Genomics (Vol. 18, Issue 1). Springer Science and Business Media LLC. https://doi.org/10.1186/s12864-017-3743-1
dc.identifier.doi10.1186/s12864-017-3743-1
dc.identifier.orcidhttps://orcid.org/0000-0002-1629-5726
dc.identifier.orcidhttps://orcid.org/0000-0003-4905-7852
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11027
dc.languageEnglish
dc.language.isoen_US
dc.publisherBMC
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHyperplasia
dc.subjectHypertrophy
dc.subjectNRF2
dc.subjectmTOR
dc.subjectRegeneration
dc.subjectRNAseq
dc.subjectACTIVATED PROTEIN-KINASE
dc.subjectNF-KAPPA-B
dc.subjectCROSS-TALK
dc.subjectC-MYC
dc.subjectMETABOLIC-RESPONSE
dc.subjectFATTY-ACIDS
dc.subjectPERFORMANCE
dc.subjectPATHWAYS
dc.subjectSREBPS
dc.subjectBiotechnology & Applied Microbiology
dc.subjectGenetics & Heredity
dc.titleGrowth and stress response mechanisms underlying post- feeding regenerative organ growth in the Burmese pythonen_US
dc.typeArticle
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