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Knockdown of a Zebrafish Aryl Hydrocarbon Receptor Repressor (AHRRa) Affects Expression of Genes Related to Photoreceptor Development and Hematopoiesis

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dc.contributor.authorAluru, Neelakanteswar
dc.contributor.authorJenny, Matthew J.
dc.contributor.authorHahn, Mark E.
dc.contributor.otherWoods Hole Oceanographic Institution
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:04:59Z
dc.date.available2023-09-28T19:04:59Z
dc.date.issued2014
dc.description.abstractThe aryl hydrocarbon receptor repressor (AHRR) is a transcriptional repressor of aryl hydrocarbon receptor (AHR) and hypoxia-inducible factor (HIF) and is regulated by an AHR-dependent mechanism. Zebrafish (Danio rerio) possess two AHRR paralogs; AHRRa regulates constitutive AHR signaling during development, whereas AHRRb regulates polyaromatic hydrocarbon-induced gene expression. However, little is known about the endogenous roles and targets of AHRRs. The objective of this study was to elucidate the role of AHRRs during zebrafish development using a loss-of-function approach followed by gene expression analysis. Zebrafish embryos were microinjected with morpholino oligonucleotides against AHRRa or AHRRb to knockdown AHRR protein expression. At 72 h postfertilization (hpf), microarray analysis revealed that the expression of 279 and 116 genes was altered by knockdown of AHRRa and AHRRb, respectively. In AHRRa-morphant embryos, 97 genes were up-regulated and 182 genes were down-regulated. Among the down-regulated genes were several related to photoreceptor function, including cone-specific genes such as several opsins (opn1sw1, opn1sw2, opn1mw1, and opn1lw2), phosphodiesterases (pde6H and pde6C), retinol binding protein (rbp4l), phosducin, and arrestins. Down-regulation was confirmed by RT-PCR and with samples from an independent experiment. The four genes tested (opn1sw1, pde6H, pde6C, and arr3b) were not inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin. AHRRa knockdown also caused up-regulation of embryonic hemoglobin (hbbe3), suggesting a role for AHRR in regulating hematopoiesis. Knockdown of AHRRb caused up-regulation of 31 genes and down-regulation of 85 genes, without enrichment for any specific biological process. Overall, these results suggest that AHRRs may have important roles in development, in addition to their roles in regulating xenobiotic signaling.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationAluru, N., Jenny, M. J., & Hahn, M. E. (2014). Knockdown of a Zebrafish Aryl Hydrocarbon Receptor Repressor (AHRRa) Affects Expression of Genes Related to Photoreceptor Development and Hematopoiesis. In Toxicological Sciences (Vol. 139, Issue 2, pp. 381–395). Oxford University Press (OUP). https://doi.org/10.1093/toxsci/kfu052
dc.identifier.doi10.1093/toxsci/kfu052
dc.identifier.orcidhttps://orcid.org/0000-0003-4358-2082
dc.identifier.orcidhttps://orcid.org/0000-0001-5974-6454
dc.identifier.orcidhttps://orcid.org/0000-0002-1834-2629
dc.identifier.urihttps://ir.ua.edu/handle/123456789/10819
dc.languageEnglish
dc.language.isoen_US
dc.publisherOxford University Press
dc.subjectmicroarrays
dc.subjectmorpholino oligonucleotides
dc.subjectopsins
dc.subjectcones
dc.subjectdevelopment
dc.subjectrepressor
dc.subjectzebrafish
dc.subjectaryl hydrocarbon receptor
dc.subjectdioxin
dc.subjectTCDD
dc.subjectAHR
dc.subjectAHRR
dc.subject2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
dc.subjectDEGENERATION
dc.subjectINDUCIBILITY
dc.subjectFIBRINOGEN
dc.subjectEXPANSION
dc.subjectPROTEINS
dc.subjectNETWORKS
dc.subjectSYSTEM
dc.subjectGROWTH
dc.subjectToxicology
dc.titleKnockdown of a Zebrafish Aryl Hydrocarbon Receptor Repressor (AHRRa) Affects Expression of Genes Related to Photoreceptor Development and Hematopoiesisen_US
dc.typeArticle
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