Phage Genetic Engineering Using CRISPR-Cas Systems

Hatoum-Aslan, Asma

Phage Genetic Engineering Using CRISPR-Cas Systems

dc.contributor.author	Hatoum-Aslan, Asma
dc.contributor.other	University of Alabama Tuscaloosa
dc.date.accessioned	2023-10-02T15:16:48Z
dc.date.available	2023-10-02T15:16:48Z
dc.date.issued	2018
dc.description.abstract	Since their discovery over a decade ago, the class of prokaryotic immune systems known as CRISPR-Cas have afforded a suite of genetic tools that have revolutionized research in model organisms spanning all domains of life. CRISPR-mediated tools have also emerged for the natural targets of CRISPR-Cas immunity, the viruses that specifically infect bacteria, or phages. Despite their status as the most abundant biological entities on the planet, the majority of phage genes have unassigned functions. This reality underscores the need for robust genetic tools to study them. Recent reports have demonstrated that CRISPR-Cas systems, specifically the three major types (I, II, and III), can be harnessed to genetically engineer phages that infect diverse hosts. Here, the mechanisms of each of these systems, specific strategies used, and phage editing efficacies will be reviewed. Due to the relatively wide distribution of CRISPR-Cas systems across bacteria and archaea, it is anticipated that these immune systems will provide generally applicable tools that will advance the mechanistic understanding of prokaryotic viruses and accelerate the development of novel technologies based on these ubiquitous organisms.	en_US
dc.format.medium	electronic
dc.format.mimetype	application/pdf
dc.identifier.citation	Hatoum-Aslan, A. (2018). Phage Genetic Engineering Using CRISPR–Cas Systems. In Viruses (Vol. 10, Issue 6, p. 335). MDPI AG. https://doi.org/10.3390/v10060335
dc.identifier.doi	10.3390/v10060335
dc.identifier.orcid	https://orcid.org/0000-0003-2395-8900
dc.identifier.uri	https://ir.ua.edu/handle/123456789/12569
dc.language	English
dc.language.iso	en_US
dc.publisher	MDPI
dc.rights.license	Attribution 4.0 International (CC BY 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	CRISPR-Cas
dc.subject	bacteriophage
dc.subject	genome editing
dc.subject	phage genetics
dc.subject	BACTERIAL IMMUNE-SYSTEM
dc.subject	GUIDED SURVEILLANCE COMPLEX
dc.subject	STREPTOCOCCUS-THERMOPHILUS
dc.subject	BACTERIOPHAGE-T4 GENOME
dc.subject	LYSOGENIC CONVERSION
dc.subject	ANTIVIRAL DEFENSE
dc.subject	CRYSTAL-STRUCTURE
dc.subject	ESCHERICHIA-COLI
dc.subject	RNA MATURATION
dc.subject	SEED SEQUENCE
dc.subject	Virology
dc.title	Phage Genetic Engineering Using CRISPR-Cas Systems	en_US
dc.type	Review
dc.type	text