Using linkage maps to correct and scaffold de novo genome assemblies: methods, challenges, and computational tools

dc.contributor.authorFierst, Janna L.
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T22:05:21Z
dc.date.available2023-09-28T22:05:21Z
dc.date.issued2015
dc.description.abstractModern high-throughput DNA sequencing has made it possible to inexpensively produce genome sequences, but in practice many of these draft genomes are fragmented and incomplete. Genetic linkage maps based on recombination rates between physical markers have been used in biology for over 100 years and a linkage map, when paired with a de novo sequencing project, can resolve mis-assemblies and anchor chromosome-scale sequences. Here, I summarize the methodology behind integrating de novo assemblies and genetic linkage maps, outline the current challenges, review the available software tools, and discuss new mapping technologies.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationFierst, J. L. (2015). Using linkage maps to correct and scaffold de novo genome assemblies: methods, challenges, and computational tools. In Frontiers in Genetics (Vol. 6). Frontiers Media SA. https://doi.org/10.3389/fgene.2015.00220
dc.identifier.doi10.3389/fgene.2015.00220
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12329
dc.languageEnglish
dc.language.isoen_US
dc.publisherFrontiers
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectFACTOR-BASED VECTOR
dc.subjectGENETIC MAPS
dc.subjectIN-VITRO
dc.subjectCHROMATIN INTERACTIONS
dc.subjectRECOMBINANT INBREDS
dc.subjectMICROBIAL GENOMES
dc.subjectHUMAN DNA
dc.subjectSHOTGUN
dc.subjectCONSTRUCTION
dc.subjectDROSOPHILA
dc.subjectGenetics & Heredity
dc.titleUsing linkage maps to correct and scaffold de novo genome assemblies: methods, challenges, and computational toolsen_US
dc.typeReview
dc.typetext
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