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Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

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dc.contributor.authorIslam, Nazrul
dc.contributor.authorKim, Yonghyun
dc.contributor.authorRoss, Julia M.
dc.contributor.authorMarten, Mark R.
dc.contributor.otherUniversity of Maryland Baltimore County
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:19:31Z
dc.date.available2023-09-28T19:19:31Z
dc.date.issued2014
dc.description.abstractBackground: The biofilm forming bacterium Staphylococcus aureus is responsible for maladies ranging from severe skin infection to major diseases such as bacteremia, endocarditis and osteomyelitis. A flow displacement system was used to grow S. aureus biofilms in four physiologically relevant fluid shear rates (50, 100, 500 and 1000 s(-1)) to identify proteins that are associated with biofilm. Results: Global protein expressions from the membrane and cytosolic fractions of S. aureus biofilm cells grown under the above shear rate conditions are reported. Sixteen proteins in the membrane-enriched fraction and eight proteins in the cytosolic fraction showed significantly altered expression (p < 0.05) under increasing fluid shear. These 24 proteins were identified using nano-LC-ESI-MS/MS. They were found to be associated with various metabolic functions such as glycolysis / TCA pathways, protein synthesis and stress tolerance. Increased fluid shear stress did not influence the expression of two important surface binding proteins: fibronectin-binding and collagen-binding proteins. Conclusions: The reported data suggest that while the general metabolic function of the sessile bacteria is minimal under high fluid shear stress conditions, they seem to retain the binding capacity to initiate new infections.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationIslam, N., Kim, Y., Ross, J. M., & Marten, M. R. (2014). Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions. In Proteome Science (Vol. 12, Issue 1, p. 21). Springer Science and Business Media LLC. https://doi.org/10.1186/1477-5956-12-21
dc.identifier.doi10.1186/1477-5956-12-21
dc.identifier.orcidhttps://orcid.org/0000-0001-6344-1258
dc.identifier.orcidhttps://orcid.org/0000-0002-9276-8388
dc.identifier.orcidhttps://orcid.org/0000-0002-1863-8956
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11300
dc.languageEnglish
dc.language.isoen_US
dc.publisherBMC
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBiofilm
dc.subjectStaphylococcus aureus
dc.subjectFlow chamber
dc.subjectShear stress
dc.subjectProteomics
dc.subjectBACTERIAL MOONLIGHTING PROTEINS
dc.subjectFIBRONECTIN-BINDING PROTEINS
dc.subjectCOLLAGEN ADHESIN
dc.subjectVIRULENCE
dc.subjectIDENTIFICATION
dc.subjectMECHANISMS
dc.subjectRESISTANCE
dc.subjectADHERENCE
dc.subjectKINETICS
dc.subjectSURVIVAL
dc.subjectBiochemical Research Methods
dc.titleProteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditionsen_US
dc.typeArticle
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