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Electron Spin Relaxation of Photoexcited Porphyrin in Water-Glycerol Glass

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dc.contributor.authorSannikova, Natalya
dc.contributor.authorTimofeev, Ivan
dc.contributor.authorBagryanskaya, Elena
dc.contributor.authorBowman, Michael
dc.contributor.authorFedin, Matvey
dc.contributor.authorKrumkacheva, Olesya
dc.contributor.otherInternational Tomography Center, Siberian Branch of Russian Academy of Sciences
dc.contributor.otherRussian Academy of Sciences
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:17:26Z
dc.date.available2023-09-28T19:17:26Z
dc.date.issued2020
dc.description.abstractRecently, the photoexcited triplet state of porphyrin was proposed as a promising spin-label for pulsed dipolar electron paramagnetic resonance (EPR). Herein, we report the factors that determine the electron spin echo dephasing of the photoexcited porphyrin in a water-glycerol matrix. The electron spin relaxation of a water-soluble porphyrin was measured by Q-band EPR, and the temperature dependence and the effect of solvent deuteration on the relaxation times were studied. The phase memory relaxation rate (1/T-m) is noticeably affected by solvent nuclei and is substantially faster in protonated solvents than in deuterated solvents. The T-m is as large as 13-17 mu s in deuterated solvent, potentially expanding the range of distances available for measurement by dipole spectroscopy with photoexcited porphyrin. The 1/T-m depends linearly on the degree of solvent deuteration and can be used to probe the environment of a porphyrin in or near a biopolymer, including the solvent accessibility of porphyrins used in photodynamic therapy. We characterized the noncovalent binding of porphyrin to human serum albumin (HSA) from 1/T-m and electron spin echo envelope modulation (ESEEM) and found that porphyrin is quite exposed to solvent on the surface of HSA. The 1/T-m and ESEEM are equally effective and provide complementary methods to determine the solvent accessibility of a porphyrin bound to protein or to determine the location of the porphyrin.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationSannikova, N., Timofeev, I., Bagryanskaya, E., Bowman, M., Fedin, M., & Krumkacheva, O. (2020). Electron Spin Relaxation of Photoexcited Porphyrin in Water—Glycerol Glass. In Molecules (Vol. 25, Issue 11, p. 2677). MDPI AG. https://doi.org/10.3390/molecules25112677
dc.identifier.doi10.3390/molecules25112677
dc.identifier.orcidhttps://orcid.org/0000-0002-0777-5103
dc.identifier.orcidhttps://orcid.org/0000-0002-0537-5755
dc.identifier.orcidhttps://orcid.org/0000-0003-0057-383X
dc.identifier.orcidhttps://orcid.org/0000-0003-0688-8947
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11249
dc.languageEnglish
dc.language.isoen_US
dc.publisherMDPI
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectspin labels
dc.subjectEPR spectroscopy
dc.subjectporphyrin
dc.subjectelectron spin relaxation
dc.subjectdistance measurements
dc.subjectDEER
dc.subjectPELDOR
dc.subjectDISTANCE MEASUREMENTS
dc.subjectTRIPLET-STATE
dc.subjectDIPOLAR SPECTROSCOPY
dc.subjectESEEM SPECTROSCOPY
dc.subjectEPR
dc.subjectECHO
dc.subjectMOLECULES
dc.subjectRESONANCE
dc.subjectPEPTIDES
dc.subjectRADICALS
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry, Multidisciplinary
dc.titleElectron Spin Relaxation of Photoexcited Porphyrin in Water-Glycerol Glassen_US
dc.typeArticle
dc.typetext

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