Caffeine and Theophylline Inhibit beta-Galactosidase Activity and Reduce Expression in Escherichia coli

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dc.contributor.authorHorne, Jesse
dc.contributor.authorBeddingfield, Elizabeth
dc.contributor.authorKnapp, Madison
dc.contributor.authorMitchell, Stephanie
dc.contributor.authorCrawford, Logan
dc.contributor.authorMills, Shelby Brooks
dc.contributor.authorWrist, Alexandra
dc.contributor.authorZhang, Shuyuan
dc.contributor.authorSummers, Ryan M.
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-09-28T19:17:02Z
dc.date.available2023-09-28T19:17:02Z
dc.date.issued2020
dc.description.abstractThe beta-galactosidase enzyme is a common reporter enzyme that has been used extensively in microbiological and synthetic biology research. Here, we demonstrate that caffeine and theophylline, common natural methylxanthine products found in many foods and pharmaceuticals, negatively impact both the expression and activity of beta-galactosidase in Escherichia coli. The beta-galactosidase activity in E. coli grown with increasing concentrations of caffeine and theophylline was reduced over sixfold in a dose-dependent manner. We also observed decreasing lacZ mRNA transcript levels with increasing methylxanthine concentrations in the growth media. Similarly, caffeine and theophylline inhibit the activity of the purified beta-galactosidase enzyme, with an approximately 1.7-fold increase in K-M toward o-nitrophenyl-beta-galactoside and a concomitant decrease in nu(max). The authors recommend the use of alternative reporter systems when such methylxanthines are expected to be present.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationHorne, J., Beddingfield, E., Knapp, M., Mitchell, S., Crawford, L., Mills, S. B., Wrist, A., Zhang, S., & Summers, R. M. (2020). Caffeine and Theophylline Inhibit β-Galactosidase Activity and Reduce Expression in Escherichia coli. In ACS Omega (Vol. 5, Issue 50, pp. 32250–32255). American Chemical Society (ACS). https://doi.org/10.1021/acsomega.0c03909
dc.identifier.doi10.1021/acsomega.0c03909
dc.identifier.orcidhttps://orcid.org/0000-0002-5941-9944
dc.identifier.orcidhttps://orcid.org/0000-0003-1871-5445
dc.identifier.orcidhttps://orcid.org/0000-0003-1358-6605
dc.identifier.urihttps://ir.ua.edu/handle/123456789/11238
dc.languageEnglish
dc.language.isoen_US
dc.publisherAmerican Chemical Society
dc.subjectYEAST SACCHAROMYCES-CEREVISIAE
dc.subjectPROTEINS
dc.subjectDOCKING
dc.subjectChemistry, Multidisciplinary
dc.titleCaffeine and Theophylline Inhibit beta-Galactosidase Activity and Reduce Expression in Escherichia colien_US
dc.typeArticle
dc.typetext
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