DNA methylation in adults and during development of the self-fertilizing mangrove rivulus, Kryptolebias marmoratus

dc.contributor.authorFellous, Alexandre
dc.contributor.authorLabed-Veydert, Tiphaine
dc.contributor.authorLocrel, Melodie
dc.contributor.authorVoisin, Anne-Sophie
dc.contributor.authorEarley, Ryan L.
dc.contributor.authorSilvestre, Frederic
dc.contributor.otherUniversity of Namur
dc.contributor.otherUniversity of Alabama Tuscaloosa
dc.date.accessioned2023-10-02T15:16:46Z
dc.date.available2023-10-02T15:16:46Z
dc.date.issued2018
dc.description.abstractIn addition to genetic variation, epigenetic mechanisms such as DNA methylation might make important contributions to heritable phenotypic diversity in populations. However, it is often difficult to disentangle the contributions of genetic and epigenetic variation to phenotypic diversity. Here, we investigated global DNA methylation and mRNA expression of the methylation-associated enzymes during embryonic development and in adult tissues of one natural isogenic lineage of mangrove rivulus fish, Kryptolebias marmoratus. Being the best-known self-fertilizing hermaphroditic vertebrate affords the opportunity to work with genetically identical individuals to examine, explicitly, the phenotypic effects of epigenetic variance. Using the LUminometric Methylation Assay (LUMA), we described variable global DNA methylation at CpG sites in adult tissues, which differed significantly between hermaphrodite ovotestes and male testes (79.6% and 87.2%, respectively). After fertilization, an immediate decrease in DNA methylation occurred to 15.8% in gastrula followed by re-establishment to 70.0% by stage 26 (liver formation). Compared to zebrafish, at the same embryonic stages, this reprogramming event seems later, deeper, and longer. Furthermore, genes putatively encoding DNA methyltransferases (DNMTs), Ten-Eleven Translocation (TET), and MeCP2 proteins showed specific regulation in adult gonad and brain, and also during early embryogenesis. Their conserved domains and expression profiles suggest that these proteins play important roles during reproduction and development. This study raises questions about mangrove rivulus' peculiar reprogramming period in terms of epigenetic transmission and physiological adaptation of individuals to highly variable environments. In accordance with the general-purpose genotype model, epigenetic mechanisms might allow for the expression of diverse phenotypes among genetically identical individuals. Such phenotypes might help to overcome environmental challenges, making the mangrove rivulus a valuable vertebrate model for ecological epigenetic studies. The mangrove rivulus, Kryptolebias marmoratus, is the best-known self-fertilizing hermaphroditic vertebrate that allows to work with genetically identical individuals to examine, explicitly, the phenotypic effects of epigenetic variance. The reprogramming event is later, more dramatic and longer than in other described vertebrates. High evolutionary conservation and expression patterns of DNMT, TET, and MeCP2 proteins in K. marmoratus suggest biological roles for each member in gametogenesis and development.en_US
dc.format.mediumelectronic
dc.format.mimetypeapplication/pdf
dc.identifier.citationFellous, A., Labed‐Veydert, T., Locrel, M., Voisin, A., Earley, R. L., & Silvestre, F. (2018). <scp>DNA</scp> methylation in adults and during development of the self‐fertilizing mangrove rivulus, Kryptolebias marmoratus. In Ecology and Evolution (Vol. 8, Issue 12, pp. 6016–6033). Wiley. https://doi.org/10.1002/ece3.4141
dc.identifier.doi10.1002/ece3.4141
dc.identifier.orcidhttps://orcid.org/0000-0003-1296-183X
dc.identifier.urihttps://ir.ua.edu/handle/123456789/12568
dc.languageEnglish
dc.language.isoen_US
dc.publisherWiley
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectdevelopment
dc.subjectDNA methylation
dc.subjectKryptolebias marmoratus
dc.subjectLUMA
dc.subjectreprogramming
dc.subjectself-fertilization
dc.subjectMEDAKA ORYZIAS-LATIPES
dc.subjectMAMMALIAN DEVELOPMENT
dc.subjectLUMINOMETRIC METHYLATION
dc.subjectINBREEDING DEPRESSION
dc.subjectHERMAPHRODITIC FISH
dc.subjectTET FAMILY
dc.subjectPHENOTYPIC PLASTICITY
dc.subjectEMBRYONIC-DEVELOPMENT
dc.subjectCYTOSINE METHYLATION
dc.subjectMOLECULAR EVOLUTION
dc.subjectEcology
dc.subjectEvolutionary Biology
dc.titleDNA methylation in adults and during development of the self-fertilizing mangrove rivulus, Kryptolebias marmoratusen_US
dc.typeArticle
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