(American Society for Microbiology, 1970-09-01) Hayes, A. Wallace; Wyatt, Elwanda P.; King, Patricia A.
Rubratoxin B can be produced in a semisynthetic medium by Penicillium rubrum under varying environmental and nutritional conditions. Maximum production (552.0 mg/500 ml) was obtained with P. rubrum NRRL A-11785 grown in stationary cultures of Mosseray's simplified Raulin solution supplemented with 2.5% malt extract broth at ambient temperature. Zinc is required at levels of at least 0.4 mg per liter. In the absence of iron sulfate, there was a 50-fold reduction in rubratoxin B production but not in growth. No toxin was produced by this isolate in 5- or 7-liter fermentors.
A time-base encoding system is described which can be adapted
to high precision without reaching a point of inaccurate digital
representation of the reference voltage. The system uses a sinusoidal reference voltage and a counter which is capable of accumulating pulses in such a way that the encoder output is an accurate
digital representation of the reference during the encoding interval.
There is no need for sample and hold circuitry in this particular
encoder. The design of the comparator, the clock inhibit circuit,
and the nonlinear counter are discussed.