Assembly and annotation of diverse genomes using long-read sequencing technologies

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dc.contributor Hatoum-Aslan, Asma
dc.contributor Kocot, Kevin M.
dc.contributor McKain, Michael R.
dc.contributor.advisor Fierst, Janna L.
dc.contributor.advisor Akob, Denise M.
dc.contributor.author Sutton, John Michael
dc.date.accessioned 2021-07-07T14:37:12Z
dc.date.available 2021-07-07T14:37:12Z
dc.date.issued 2021
dc.identifier.other u0015_0000001_0003833
dc.identifier.other Sutton_alatus_0004D_14439
dc.identifier.uri http://ir.ua.edu/handle/123456789/7912
dc.description Electronic Thesis or Dissertation
dc.description.abstract The creation of high-quality reference genomes has become increasingly feasible for researchers with the democratization of DNA sequencing brought on by third generation sequencing platforms. New tools are now in the hands of researchers that may have been previously priced out of generating quality genome assemblies. With the steady increase in research groups generating sequence data, the development of rigorous guidelines that are widely applicable across the tree of life are necessary for maximizing the potential of sequence data generated. Here we present a study of simulated sequence data for the model organisms Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Arabidopsis thaliana. We benchmarked several approaches to managing long-read data for de novo genome assembly to develop assembly approach recommendations. From these benchmarks, we created four nematode genomes from strains of C. remanei and C. latens as proof of concept. Additional studies demonstrated the downstream utilization of quality genome assemblies in bacterial isolates that consume acetylene as a primary carbon and energy source. Acetylenotrophy has been documented in a wide range of environmental samples as well as diverse aerobic and anaerobic bacterial isolates. The generation of genomic resources for these isolates allowed for characterization of the organisms, accurate genome-inferred taxonomicplacement and investigation of the metabolic pathways used to transform acetylene. These studies identified the first organisms capable of acetylenotrophic and diazotrophic growth, the anaerobic Syntrophotalea acetylenivorans SFB93T, and the aerobic Bradyrhizobium sp. strain I71. While both capable of consuming acetylene, these studies and others have noted distinct diversity of their causative Acetylene Hydratase enzymes. Further comparison of the genomes of other acetylenotrophs generated in these studies and elsewhere, may give insight into the diversity of this metabolism as well as its evolutionary origin.
dc.format.extent 215 p.
dc.format.medium electronic
dc.format.mimetype application/pdf
dc.language English
dc.language.iso en_US
dc.publisher University of Alabama Libraries
dc.relation.ispartof The University of Alabama Electronic Theses and Dissertations
dc.relation.ispartof The University of Alabama Libraries Digital Collections
dc.relation.haspart Supplementary materials include an Excel spreadsheet containing supplemental data for Chapter 2.
dc.relation.hasversion born digital
dc.rights All rights reserved by the author unless otherwise indicated.
dc.subject.other Biology
dc.subject.other Microbiology
dc.subject.other Bioinformatics
dc.title Assembly and annotation of diverse genomes using long-read sequencing technologies
dc.type thesis
dc.type text
etdms.degree.department University of Alabama. Department of Biological Sciences
etdms.degree.discipline Biological Sciences
etdms.degree.grantor The University of Alabama
etdms.degree.level doctoral
etdms.degree.name Ph.D.


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