Characterization of the regions surrounding zooA-zif and lss-lif

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dc.contributor Simmonds, Robin S.
dc.contributor LeBlanc, Paul A.
dc.contributor Heath, Harry E.
dc.contributor Heath, Lucie S.
dc.contributor Churchill, Perry F.
dc.contributor.advisor Sloan, Gary L. Gargis, Amy Shirley 2017-02-28T22:26:58Z 2017-02-28T22:26:58Z 2010
dc.identifier.other u0015_0000001_0000284
dc.identifier.other Gargis_alatus_0004D_10289
dc.description Electronic Thesis or Dissertation
dc.description.abstract Staphylococcus simulans biovar staphylolyticus produces the staphylolytic enzyme lysostaphin and it is the only known lysostaphin producer within the species. Genes with similarity to the lysostaphin gene (lss) and the gene for lysostaphin resistance (lif) have been characterized, including the chromosomally encoded streptococcolytic enzyme zoocin A (zooA) and its immunity factor (zif) from Streptococcus equi subsp. zooepidemicus strain 4881. Both zooA - zif and lss - lif are divergently transcribed and the similarity in genetic arrangement between these loci generated the hypothesis that zif and zooA were acquired by horizontal gene transfer. Initially, twenty - four S. equi subsp. zooepidemicus strains were analyzed to determine the prevalence and acquisition of the zooA - zif locus within the subspecies. While zooA - zif is not common within the subspecies, PFGE and RAPD showed that the 24 strains were genetically diverse. Sequences derived from strain 4881 revealed that zooA - zif are flanked by two transposon like sequences and are integrated into the chromosome adjacent to the gene flaR. A comparison of the flaR region of S. equi subsp. zooepidemicus strains 4881, 9g, H70 and MGCS10565, as well as the related S. equi subsp. equi strain 4047, suggested that flaR marks a region of genome plasticity in these streptococci. S. simulans bv. staphylolyticus contains five plasmids designated pACK1 - pACK5. lss and lif are encoded on the largest plasmid, pACK1. In order to investigate the regions flanking lss and lif, as well as to understand the relationship between pACK1 and pACK3 within the strain, the sequences of pACK1 (55171 bp) and pACK3 (28613 bp) were determined. Comparison of the two plasmids revealed that they are virtually identical within an approximately 28 kb common region. Sequences flanking the common region contain IS431 elements and direct repeats mark where the two plasmid sequences diverge, providing evidence that pACK1 was derived from pACK3 by insertion of sequences unique to pACK1 into pACK3. lss and lif are located within the region unique to pACK1 and, as with zooA - zif, are flanked by two transposon like sequences. The presence of flanking transposons near zooA - zif and lss - lif suggests that these organisms may have received these genes by horizontal gene transfer.
dc.format.extent 69 p.
dc.format.medium electronic
dc.format.mimetype application/pdf
dc.language English
dc.language.iso en_US
dc.publisher University of Alabama Libraries
dc.relation.ispartof The University of Alabama Electronic Theses and Dissertations
dc.relation.ispartof The University of Alabama Libraries Digital Collections
dc.relation.hasversion born digital
dc.rights All rights reserved by the author unless otherwise indicated.
dc.subject.other Biology, Microbiology
dc.title Characterization of the regions surrounding zooA-zif and lss-lif
dc.type thesis
dc.type text University of Alabama. Dept. of Biological Sciences Biological Sciences The University of Alabama doctoral Ph.D.

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