Browsing by Author "Kim, Eunjee"

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    Endoplasmic reticulum stress impairment in the spinal dorsal horn of a neuropathic pain model
    (Nature Portfolio, 2015) Zhang, Enji; Yi, Min-Hee; Shin, Nara; Baek, Hyunjung; Kim, Sena; Kim, Eunjee; Kwon, Kisang; Lee, Sunyeul; Kim, Hyun-Woo; Bae, Yong Chul; Kim, Yonghyun; Kwon, O. -Yu; Lee, Won Hyung; Kim, Dong Woon; Chungnam National University; Chungnam National University Hospital; Kyungpook National University; Yanbian University; University of Alabama Tuscaloosa
    Endoplasmic reticulum (ER) stress has been implicated in neurodegenerative diseases, but its role in neuropathic pain remains unclear. In this study, we examined the ER stress and the unfolded protein response (UPR) activation in a L5 spinal nerve ligation (SNL)-induced rat neuropathic pain model. SNL-induced neuropathic pain was assessed behaviorally using the CatWalk system, and histologically with microglial activation in the dorsal spinal horn. L5 SNL induced BIP upregulation in the neuron of superficial laminae of dorsal spinal horn. It also increased the level of ATF6 and intracellular localization into the nuclei in the neurons. Moreover, spliced XBP1 was also markedly elevated in the ipsilateral spinal dorsal horn. The PERK-elF2 pathway was activated in astrocytes of the spinal dorsal horn in the SNL model. In addition, electron microscopy revealed the presence of swollen cisternae in the dorsal spinal cord after SNL. Additionally, inhibition of the ATF6 pathway by intrathecal treatment with ATF6 siRNA reduced pain behaviors and BIP expression in the dorsal horn. The results suggest that ER stress might be involved in the induction and maintenance of neuropathic pain. Furthermore, a disturbance in UPR signaling may render the spinal neurons vulnerable to peripheral nerve injury or neuropathic pain stimuli.
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    Expression of PGC1 alpha in glioblastoma multiforme patients
    (Spandidos, 2017) Cho, Sang Yeon; Kim, Seon-Hwan; Yi, Min-Hee; Zhang, Enji; Kim, Eunjee; Park, Jisoo; Jo, Eun-Kyeong; Lee, Young Ho; Park, Min Soo; Kim, Yonghyun; Park, Jongsun; Kim, Dong Woon; Chungnam National University; Chungnam National University Hospital; Yanbian University; University of Alabama Tuscaloosa
    Peroxisome proliferator-activated receptor. coactivator 1 alpha (PGC1 alpha) is a key modulator of mitochondrial biogenesis. It is a coactivator of multiple transcription factors and regulates metabolic processes. However, little is known about the expression and function of PGC1 alpha in glioblastoma multiforme (GBM), the most prevalent and invasive type of brain tumor. The purpose of the present study was to investigate the biological function, localization and expression of PGC1 alpha in GBM. It was observed that PGC1 alpha expression is increased in the tumor cells, and a higher level of expression was observed in the mitochondria. Bioinformatics analyses identified that metabolic and mitochondrial genes were highly expressed in GBM cells, with a high PGC1 alpha mRNA expression. Notably, mitochondrial function-associated genes were highly expressed in cells alongside high PGC1 alpha expression. Collectively, the results of the present study indicate that PGC1 alpha is associated with mitochondrial dysfunction in GBM and may have a role in tumor pathogenesis and progression.