Research and Publications - Department of Chemical & Biological Engineering

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Browsing Research and Publications - Department of Chemical & Biological Engineering by Author "Arizona State University"

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    Controlling Electron Transfer between the Two Cofactor Chains of Photosystem I by the Redox State of One of Their Components
    (Cell Press, 2015) Santabarbara, Stefano; Bullock, Bradford; Rappaport, Fabrice; Redding, Kevin E.; Arizona State University; Arizona State University-Tempe; UDICE-French Research Universities; Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS); CNRS - National Institute for Biology (INSB); Consiglio Nazionale delle Ricerche (CNR); Istituto di Biofisica (IBF-CNR); University of Alabama Tuscaloosa
    Two functional electron transfer (ET) chains, related by a pseudo-C-2 symmetry, are present in the reaction center of photosystem I (PSI). Due to slight differences in the environment around the cofactors of the two branches, there are differences in both the kinetics of ET and the proportion of ET that occurs on the two branches. The strongest evidence that this is indeed the case relied on the observation that the oxidation rates of the reduced phylloquinone (PhQ) cofactor differ by an order of magnitude. Site-directed mutagenesis of residues involved in the respective PhQ-binding sites resulted in a specific alteration of the rates of semiquinone oxidation. Here, we show that the PsaA-F689N mutation results in an similar to 100-fold decrease in the observed rate of PhQ(A)(-) oxidation. This is the largest change of PhQ(A)(-) oxidation kinetics observed so far for a single-point mutation, resulting in a lifetime that exceeds that of the terminal electron donor, P-700(+). This situation allows a second photochemical charge separation event to be initiated before PhQ(A)(-) has decayed, thereby mimicking in PSI a situation that occurs in type II reaction centers. The results indicate that the presence of PhQ(A)(-) does not impact the overall quantum yield and leads to an almost complete redistribution of the fractional utilization of the two functional ET chains, in favor of the one that does not bear the charged species. The evolutionary implications of these results are also briefly discussed.
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    The genomes of two key bumblebee species with primitive eusocial organization
    (BMC, 2015) Sadd, Ben M.; Barribeau, Seth M.; Bloch, Guy; de Graaf, Dirk C.; Dearden, Peter; Elsik, Christine G.; Gadau, Juergen; Grimmelikhuijzen, Cornelis J. P.; Hasselmann, Martin; Lozier, Jeffrey D.; Robertson, Hugh M.; Smagghe, Guy; Stolle, Eckart; Van Vaerenbergh, Matthias; Waterhouse, Robert M.; Bornberg-Bauer, Erich; Klasberg, Steffen; Bennett, Anna K.; Camara, Francisco; Guigo, Roderic; Hoff, Katharina; Mariotti, Marco; Munoz-Torres, Monica; Murphy, Terence; Santesmasses, Didac; Amdam, Gro V.; Beckers, Matthew; Beye, Martin; Biewer, Matthias; Bitondi, Marcia M. G.; Blaxter, Mark L.; Bourke, Andrew F. G.; Brown, Mark J. F.; Buechel, Severine D.; Cameron, Rossanah; Cappelle, Kaat; Carolan, James C.; Christiaens, Olivier; Ciborowski, Kate L.; Clarke, David F.; Colgan, Thomas J.; Collins, David H.; Cridge, Andrew G.; Dalmay, Tamas; Dreier, Stephanie; du Plessis, Louis; Duncan, Elizabeth; Erler, Silvio; Evans, Jay; Falcon, Tiago; Flores, Kevin; Freitas, Flavia C. P.; Fuchikawa, Taro; Gempe, Tanja; Hartfelder, Klaus; Hauser, Frank; Helbing, Sophie; Humann, Fernanda C.; Irvine, Frano; Jermiin, Lars S.; Johnson, Claire E.; Johnson, Reed M.; Jones, Andrew K.; Kadowaki, Tatsuhiko; Kidner, Jonathan H.; Koch, Vasco; Koehler, Arian; Kraus, F. Bernhard; Lattorff, H. Michael G.; Leask, Megan; Lockett, Gabrielle A.; Mallon, Eamonn B.; Antonio, David S. Marco; Marxer, Monika; Meeus, Ivan; Moritz, Robin F. A.; Nair, Ajay; Napflin, Kathrin; Nissen, Inga; Niu, Jinzhi; Nunes, Francis M. F.; Oakeshott, John G.; Osborne, Amy; Otte, Marianne; Pinheiro, Daniel G.; Rossie, Nina; Rueppell, Olav; Santos, Carolina G.; Schmid-Hempel, Regula; Schmitt, Bjoern D.; Schulte, Christina; Simoes, Zila L. P.; Soares, Michelle P. M.; Swevers, Luc; Winnebeck, Eva C.; Wolschin, Florian; Yu, Na; Zdobnov, Evgeny M.; Aqrawi, Peshtewani K.; Blankenburg, Kerstin P.; Coyle, Marcus; Francisco, Liezl; Hernandez, Alvaro G.; Holder, Michael; Hudson, Matthew E.; Jackson, LaRonda; Jayaseelan, Joy; Joshi, Vandita; Kovar, Christie; Lee, Sandra L.; Mata, Robert; Mathew, Tittu; Newsham, Irene F.; Ngo, Robin; Okwuonu, Geoffrey; Pham, Christopher; Pu, Ling-Ling; Saada, Nehad; Santibanez, Jireh; Simmons, DeNard; Thornton, Rebecca; Venkat, Aarti; Walden, Kimberly K. O.; Wu, Yuan-Qing; Debyser, Griet; Devreese, Bart; Asher, Claire; Blommaert, Julie; Chipman, Ariel D.; Chittka, Lars; Fouks, Bertrand; Liu, Jisheng; O'Neill, Meaghan P.; Sumner, Seirian; Puiu, Daniela; Qu, Jiaxin; Salzberg, Steven L.; Scherer, Steven E.; Muzny, Donna M.; Richards, Stephen; Robinson, Gene E.; Gibbs, Richard A.; Schmid-Hempel, Paul; Worley, Kim C.; Illinois State University; Swiss Federal Institutes of Technology Domain; ETH Zurich; University of North Carolina; East Carolina University; Hebrew University of Jerusalem; Ghent University; University of Otago; University of Missouri Columbia; Georgetown University; Arizona State University; Arizona State University-Tempe; University of Copenhagen; University Hohenheim; University of Alabama Tuscaloosa; University of Illinois Urbana-Champaign; Martin Luther University Halle Wittenberg; University of Geneva; Swiss Institute of Bioinformatics; Massachusetts Institute of Technology (MIT); Harvard University; Broad Institute; University of Munster; Barcelona Institute of Science & Technology; Pompeu Fabra University; Centre de Regulacio Genomica (CRG); Ernst Moritz Arndt Universitat Greifswald; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; National Institutes of Health (NIH) - USA; NIH National Library of Medicine (NLM); University of East Anglia; Heinrich Heine University Dusseldorf; University of Cologne; Universidade de Sao Paulo; University of Edinburgh; University of London; Royal Holloway University London; University of Bristol; Commonwealth Scientific & Industrial Research Organisation (CSIRO); Trinity College Dublin; Zoological Society of London; United States Department of Agriculture (USDA); North Carolina State University; Kyoto University; Instituto Federal de Sao Paulo (IFSP); Ohio State University; Oxford Brookes University; University of Southampton; University of Leicester; Universidade Federal de Sao Carlos; Universidade Estadual Paulista; University of North Carolina Greensboro; National Centre of Scientific Research "Demokritos"; University of Munich; Baylor College of Medicine; UTMD Anderson Cancer Center; University of Chicago; Queen Mary University London; Guangzhou University; Johns Hopkins University
    Background: The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed to illuminate the evolution of highly eusocial insect societies. Bumblebees are also invaluable natural and agricultural pollinators, and there is widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects of bumblebee biology, including susceptibility to implicated population viability threats. Results: We report the high quality draft genome sequences of Bombus terrestris and Bombus impatiens, two ecologically dominant bumblebees and widely utilized study species. Comparing these new genomes to those of the highly eusocial honeybee Apis mellifera and other Hymenoptera, we identify deeply conserved similarities, as well as novelties key to the biology of these organisms. Some honeybee genome features thought to underpin advanced eusociality are also present in bumblebees, indicating an earlier evolution in the bee lineage. Xenobiotic detoxification and immune genes are similarly depauperate in bumblebees and honeybees, and multiple categories of genes linked to social organization, including development and behavior, show high conservation. Key differences identified include a bias in bumblebee chemoreception towards gustation from olfaction, and striking differences in microRNAs, potentially responsible for gene regulation underlying social and other traits. Conclusions: These two bumblebee genomes provide a foundation for post-genomic research on these key pollinators and insect societies. Overall, gene repertoires suggest that the route to advanced eusociality in bees was mediated by many small changes in many genes and processes, and not by notable expansion or depauperation.